Clinical Trial Sponsors: National Taiwan University Hospital

  Source:		NCT00173563

    		Induction of Cytokines in Human Monocytes by
     		SARS-CoV in Adults and Children

			Unknown status

			First Update September 12, 2005
			Last Update November 30, 2007

			Brief Summary
			Severe acute respiratory syndrome (SARS) is a
			new emerging infectious disease. Its pathogen
			is a newly discovered coronavirus (SARS-CoV).
			The clinical course can be classified to 3
			stages: viral replication phase, hyperimmune
			reactive phase, and pulmonary destruction
			phase. Human monocyte plays a critical role in
			the initiation of immune response in defending
			the intracellular pathogens (eg viruses).
			Monocytes can engulf viruses and present the
			viral antigens in the major histocompatibility
			(MHC) molecule to the cell surface to initiate T
			lymphocyte response. Monocytes also secrete
			various cytokines to modulate immune
			response. SARS-CoV is a mutant of animal
			virus to cause human disease and is able to
			cause unusual severe respiratory illness. It is
			suggested the unusual severe disease is due to
			the intense immune reaction. The investigators
			will harvest human monocytes from healthy
			adult and children blood donors. Monocytes
			would be cultured and infected by SARS-CoV.
			The change of viral load is monitored after
			infection. Cytokines secreted by monocytes
			after infection are also measured. The
			difference of monocyte cytokine secretion is
			compared between adults and children. The
			study is to verify the SARS-CoV infectivity of
			human monocytes and prove the unusual
			severity caused by SARS-CoV is related to
			viral-induced dysregulation of cytokine
			responses. The results may also clarify why
			adults tend to have a more severe illness
			compared with children.

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